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Cell Signaling Pitslre/Cdk11 (D88b3) Rabbit mAb
List Price
$342.39
Your Price
$342.39
Cell Signaling Pitslre/Cdk11 (D88b3) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-5524S
MFG.PART:
5524S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | PITSLRE/CDK11 (D88B3) Rabbit mAb recognizes endogenous levels of total PITSLRE/CDK11 protein. This antibody will detect the full-length (CDK11p110) and is predicted to detect the alternate transcript (CDK11p58) of PITSLRE/CDK11. The antibody is predicted to detect both PITSLREA/CDK11B and PITSLREB/CDK11A. |
| Species Reactivity | Human, Mouse, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met734 of human PITSLRE/CDK11 protein. |
| Background | PITSLRE, alternatively known as cell division kinase 11 (CDK11), is the result of duplication of the CDK11 gene (1). CDK11A and CDK11B encode nearly identical serine/threonine protein kinases, PITSLREB and PITSLREA respectively, both belonging to the p34CDC2 family of protein kinases (2). Full-length PITSLRE/CDK11 (commonly referred to as CDK11p110) is expressed ubiquitously throughout the cell cycle whereas a smaller, alternate transcript (CDK11p58), the result of internal ribosomal entry, is expressed only during the G2/M transition where it promotes centrosome maturation and spindle formation (3-5). During induction of apoptosis by Fas or TNF, or anoikis, PITSLRE/CDK11 is cleaved by caspases to generate p110C, an approximately 46 kDa protein that contains the catalytically active kinase domain of PITSLRE/CDK11 that interacts with and inhibits p21-activated kinase (PAK1) activity (6-8). Full length PITSLRE/CDK11 (CDK11p110) appears to participate in pre-mRNA splicing events. This is demonstrated by the observation that CDK11p110 interacts with numerous splicing factors including RNPS1, 9G8/SRSF7 and cyclin L and that CDK11p110 can phosphorylate and inhibit the splicing activity of 9G8/SRSF7 (9-11). |
| SKU | CSIG-5524S |
|---|---|
| Featured | No |
| Supplier Part Number | 5524S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |