Cell Signaling Phospho-Src (Ser17) Antibody

Name: Cell Signaling Phospho-Src (Ser17) Antibody
SKU: CSIG-5473S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Src (Ser17) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5473S

MFG.PART: 5473S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Src (Ser17) Antibody recognizes endogenous levels of Src protein only when phosphorylated at Ser17.
Species Reactivity	Human, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser17 of human Src protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).Protein kinase A (PKA)-dependent phosphorylation of Src at Ser17 is thought to influence multiple signaling networks (3-5). This site has also been identified in a phospho-proteomic screen for substrates of mTOR (6).

Specifications