Cell Signaling Rictor (D16h9) Rabbit mAb (Sepharose Bead Conjugate)

Name: Cell Signaling Rictor (D16h9) Rabbit mAb (Sepharose Bead Conjugate)
SKU: CSIG-5379S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Rictor (D16h9) Rabbit mAb (Sepharose Bead Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5379S

MFG.PART: 5379S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	400 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	200
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunoprecipitation: 1:20}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total rictor protein.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Leu1121 of human rictor protein.
Background	Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

Specifications