Cell Signaling Galpha(I) Antibody

Name: Cell Signaling Galpha(I) Antibody
SKU: CSIG-5290S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Galpha(I) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5290S

MFG.PART: 5290S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	40
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Gα(i) Antibody detects endogenous levels of total Gα(i) protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg100 of human Gα(i). Antibodies are purified by protein A and peptide affinity chromatography.
Background	Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines, and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).Gα(i) causes inhibition of adenylate cyclase, leading to a decrease in cellular levels of cAMP. Pertussis toxin catalyzes ADP-ribosylation of Gα(i), which inactivates the Gα(i) protein and attenuates inhibition of adenylate cyclase (4).

Specifications