Cell Signaling Cleaved Caspase-9 (Asp330) (E5z7n) Rabbit mAb

Name: Cell Signaling Cleaved Caspase-9 (Asp330) (E5z7n) Rabbit mAb
SKU: CSIG-52873S
Price: 405.01 USD

List Price $405.01 Your Price $405.01

Cell Signaling Cleaved Caspase-9 (Asp330) (E5z7n) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-52873S

MFG.PART: 52873S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	37
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp330 of human caspase-9 protein.
Background	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Specifications