Cell Signaling Phospho-Ikappabalpha (Ser32) (14d4) Rabbit mAb (Biotinylated)

Name: Cell Signaling Phospho-Ikappabalpha (Ser32) (14d4) Rabbit mAb (Biotinylated)
SKU: CSIG-5209S
Price: 481.77 USD

List Price $481.77 Your Price $481.77

Cell Signaling Phospho-Ikappabalpha (Ser32) (14d4) Rabbit mAb (Biotinylated) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5209S

MFG.PART: 5209S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	40
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	Phospho-IκBα (Ser32) (14D4) Rabbit mAb (Biotinylated) detects endogenous levels of IκBα only when phosphorylated at Ser32.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32 of human IκBα.
Background	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

Specifications