Cell Signaling Ect2 (E7v4l) Rabbit mAb

Name: Cell Signaling Ect2 (E7v4l) Rabbit mAb
SKU: CSIG-52021S
Price: 342.39 USD

List Price $342.39 Your Price $342.39

Cell Signaling Ect2 (E7v4l) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-52021S

MFG.PART: 52021S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	104
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	ECT2 (E7V4L) Rabbit mAb recognizes endogenous levels of total ECT2 protein.
Species Reactivity	Human, Mouse, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro325 of human ECT2 protein.
Background	Epithelial cell transforming sequence 2 (ECT2), a guanine nucleotide exchange factor (GEF), catalyzes the exchange of GDP for GTP. The nuclear GEF activity of ECT2 is essential for Kras-Trp53 lung tumorigenesis. This transformation requires ECT2-mediated rRNA synthesis. In addition, PKCι-dependent phosphorylation of ECT2 is necessary for the oncogenic activity of this protein (1,2). Furthermore, ECT2 has been implicated as a cancer driver gene in certain subtypes of liver cancers (3). Research studies also show that, during matrix-directed cell migration, low membrane tension stimulates the formation of caveolae at the rear end of moving cells. ECT2 is recruited to the caveolae and activates RhoA, causing F-actin organization and rear retraction (4).

Specifications