Cell Signaling Pdi (C81h6) Rabbit mAb (Alexa Fluor 488 Conjugate)

Name: Cell Signaling Pdi (C81h6) Rabbit mAb (Alexa Fluor 488 Conjugate)
SKU: CSIG-5051S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Pdi (C81h6) Rabbit mAb (Alexa Fluor 488 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5051S

MFG.PART: 5051S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M R Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit
Application/Dilution	{Immunofluorescence (Immunocytochemistry): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	PDI (C81H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total PDI protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDI.
Background	During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).

Specifications