Cell Signaling Ube1a/B Antibody

Name: Cell Signaling Ube1a/B Antibody
SKU: CSIG-4891S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Ube1a/B Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4891S

MFG.PART: 4891S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	110, 117
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:25}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	UBE1a/b Antibody detects endogenous levels of total UBE1a and UBE1b proteins.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr800 of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).

Specifications