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Cell Signaling Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2e) Xp Rabbit mAb (Alexa Fluor 647 Conjugate)
List Price
$481.77
Your Price
$481.77
Cell Signaling Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2e) Xp Rabbit mAb (Alexa Fluor 647 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-4851S
MFG.PART:
4851S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H M R Mk Mi Sc |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Immunofluorescence (Immunocytochemistry): 1:50 - 1:200, Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| Species Reactivity | Human, Mouse, Rat, Monkey, Mink, S. cerevisiae |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm. |
| Background | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5). |
| SKU | CSIG-4851S |
|---|---|
| Featured | No |
| Supplier Part Number | 4851S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |