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Cell Signaling Phospho-Mark Family (Activation Loop) Antibody
List Price
$360.57
Your Price
$360.57
Cell Signaling Phospho-Mark Family (Activation Loop) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-4836S
MFG.PART:
4836S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 80 to 95 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-MARK Family (Activation Loop) Antibody detects endogenous levels of phosphorylated MARK family members, MARK1 at threonine 215, MARK2 at threonine 208, and MARK3 at threonine 234 (A.K.A. 211 in isoforms 3-6) . This antibody does not react with MARK4. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding threonine 215 of human MARK1. Antibodies were purified by protein A and peptide affinity chromatography. |
| Background | Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimers disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7). |
| SKU | CSIG-4836S |
|---|---|
| Featured | No |
| Supplier Part Number | 4836S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |