Cell Signaling Smc1 Antibody

Name: Cell Signaling Smc1 Antibody
SKU: CSIG-4802S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Smc1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4802S

MFG.PART: 4802S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	145
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	SMC1 Antibody detects endogenous levels of total SMC1 protein.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human SMC1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).

Specifications