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Cell Signaling Hpf1 Antibody
List Price
$309.06
Your Price
$309.06
Cell Signaling Hpf1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-47590S
MFG.PART:
47590S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 40 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | HPF1 Antibody recognizes endogenous levels of total HPF1 protein. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu241 of human HPF1 protein. Antibodies are purified by peptide affinity chromatography. |
| Background | Poly (ADP-ribose) polymerases (PARPs) are a family of enzymes that catalyze ADP-ribosylation (ADPr), a reversible posttranslational modification of proteins that regulates various cellular processes. PARP-1 and PARP-2 are two well-characterized family members that catalyze ADPr on a wide range of substrates in response to DNA damage (1). Histone PARylation factor 1 (HPF1), also referred to as C4orf27, is an accessory protein to PARP1/2 that directly binds to these enzymes and alters their ADPr amino acid specificity from aspartate/glutamate to serine. (2). HPF1 is specifically required to carry out ADPr modifications on serine residues for both histone and non-histone substrates, as serine ADPr does not occur in cells lacking HPF1 (3). This is critically important, as serine has been shown to be the most abundant ADPr residue in cells affected by DNA damage (4). Mechanistically, PARP1/2 have been described as incomplete enzymes, and crystal structure analysis shows that HPF1-PARP1/2 binding confers a joint active site, which may explain the switch from aspartate/glutamate specificity to serine (2). Additionally, HPF1 knockout cells are more sensitive to PARP inhibitors, suggesting that drug manufacturers will need to take HPF1 binding into account when developing effective inhibitors for these enzymes (1). |
| SKU | CSIG-47590S |
|---|---|
| Featured | No |
| Supplier Part Number | 47590S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |