Cell Signaling P58 Primase (8d3) Rat mAb

Name: Cell Signaling P58 Primase (8d3) Rat mAb
SKU: CSIG-4726S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling P58 Primase (8d3) Rat mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4726S

MFG.PART: 4726S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Hm Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	58
Source/Isotype	Rat IgG2a
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:250}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	p58 Primase (8D3) Rat mAb detects endogenous levels of total p58 primase protein.
Species Reactivity	Human, Mouse, Rat, Hamster, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with full-length human recombinant p58 primase.
Background	Initiation of eukaryotic DNA replication is a stringently regulated process that requires the cooperation of many proteins and protein complexes to occur efficiently, at the origins of replication, and once per cell cycle. The initiation of DNA replication requires a protein complex composed of two DNA polymerase α subunits and a pair of primase subunits. Primase activity catalyzes de novo synthesis of an RNA/DNA primer (initiator DNA) on the leading and lagging strands, while polymerase activity extends the initiator DNA (1). The 48 and 58 kDa primase subunits cooperate in the synthesis of small RNA primers. p48 is the catalytically active subunit (2), while p58 couples p48 to the polymerase to allow the transfer of primers to the active site. The p58 subunit may also play a role in regulation of primer length (3,4).

Specifications