Cell Signaling Phospho-Doublecortin (Ser297) Antibody

Name: Cell Signaling Phospho-Doublecortin (Ser297) Antibody
SKU: CSIG-4605S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Doublecortin (Ser297) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4605S

MFG.PART: 4605S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H R
Sensitivity	Endogenous
Molecular Weight (kDa)	45
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser297.
Species Reactivity	Human, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser297 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Mutations in doublecortin (DCX) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

Specifications