Cell Signaling Phospho-4e-Bp1 (Thr37/46) (236b4) Rabbit mAb (Pe-Cy7 Conjugate)

Name: Cell Signaling Phospho-4e-Bp1 (Thr37/46) (236b4) Rabbit mAb (Pe-Cy7 Conjugate)
SKU: CSIG-45613S
Price: 481.77 USD

List Price $481.77 Your Price $481.77

Cell Signaling Phospho-4e-Bp1 (Thr37/46) (236b4) Rabbit mAb (Pe-Cy7 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-45613S

MFG.PART: 45613S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H M R Mk Dm
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE-Cy7® Conjugate) detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.
Species Reactivity	Human, Mouse, Rat, Monkey, D. melanogaster
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.
Background	Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Specifications