Cell Signaling Pdi (E7o2r) Mouse mAb

Name: Cell Signaling Pdi (E7o2r) Mouse mAb
SKU: CSIG-45596S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling Pdi (E7o2r) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-45596S

MFG.PART: 45596S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Source/Isotype	Mouse IgG1 kappa
Application/Dilution	{Immunohistochemistry (Paraffin): 1:400 - 1:1600, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	PDI (E7O2R) Mouse mAb recognizes endogenous levels of total PDI protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu332 of human PDI protein.
Background	During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).

Specifications