Cell Signaling Mtor (L27d4) Mouse mAb

Name: Cell Signaling Mtor (L27d4) Mouse mAb
SKU: CSIG-4517S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling Mtor (L27d4) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4517S

MFG.PART: 4517S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	289
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	mTOR (L27D4) Mouse mAb detects endogenous levels of total mTOR protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human mTOR protein.
Background	The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

Specifications