Cell Signaling Phospho-Tak1 (Thr184/187) (90c7) Rabbit mAb

Name: Cell Signaling Phospho-Tak1 (Thr184/187) (90c7) Rabbit mAb
SKU: CSIG-4508S
Price: 405.01 USD

List Price $405.01 Your Price $405.01

Cell Signaling Phospho-Tak1 (Thr184/187) (90c7) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4508S

MFG.PART: 4508S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	82
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, the antibody may cross-react with a 40 kDa band of unknown origin.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1.
Background	TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2).

Specifications