Cell Signaling Ran Antibody

Name: Cell Signaling Ran Antibody
SKU: CSIG-4462S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Ran Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4462S

MFG.PART: 4462S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Hm Mk B
Sensitivity	Endogenous
Molecular Weight (kDa)	24
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Ran Antibody detects endogenous levels of total Ran protein. The antibody does not cross-react with other small GTPases of the Ras family.
Species Reactivity	Human, Mouse, Rat, Hamster, Monkey, Bovine
Source/Purification	Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3).

Specifications