Cell Signaling Step (23e5) Mouse mAb

Name: Cell Signaling Step (23e5) Mouse mAb
SKU: CSIG-4396S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling Step (23e5) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4396S

MFG.PART: 4396S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M R
Sensitivity	Endogenous
Molecular Weight (kDa)	46, 61
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Frozen): 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	STEP (23E5) Mouse mAb detects endogenous levels of total STEP46 and STEP61 protein.
Species Reactivity	Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of rat STEP46 protein.
Background	Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimers disease (1).

Specifications