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Cell Signaling Phospho-Sapk/Jnk (Thr183/Tyr185) (81e11) Rabbit mAb (Sepharose Bead Conjugate)
List Price
$481.77
Your Price
$481.77
Cell Signaling Phospho-Sapk/Jnk (Thr183/Tyr185) (81e11) Rabbit mAb (Sepharose Bead Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-4306S
MFG.PART:
4306S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 400 µl |
| Reactivity | H M R Dm Sc |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 46, 54 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Immunoprecipitation: 1:20} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies. |
| Specificity/Sensitivity | Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of p46 and p54 SAPK/JNK only when phosphorylated at Thr183 and Tyr185. This antibody does not recognize phosphorylated p44/42 or p38 MAP kinases. |
| Species Reactivity | Human, Mouse, Rat, D. melanogaster, S. cerevisiae |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK protein. |
| Background | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| SKU | CSIG-4306S |
|---|---|
| Featured | No |
| Supplier Part Number | 4306S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |