Cell Signaling Mkk7 Antibody

Name: Cell Signaling Mkk7 Antibody
SKU: CSIG-4172S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Mkk7 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4172S

MFG.PART: 4172S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	48
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MKK7 Antibody detects endogenous levels of total MKK7. This antibody does not cross-react with SEK1/MKK4, MEK1/2 or other MAP kinase kinases.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.
Background	MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway (1,2). MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

Specifications