Cell Signaling Calpastatin Antibody

Name: Cell Signaling Calpastatin Antibody
SKU: CSIG-4146S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Calpastatin Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4146S

MFG.PART: 4146S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	120
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Calpastatin Antibody detects endogenous levels of total calpastatin protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human calpastatin. Antibodies are purified by peptide affinity chromatography.
Background	Calpain is a thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 in a calcium dependent manner (2). In particular, calpastatin binds and inhibits calpain when calcium levels are high and is released when calcium levels go down. Calpastatin contains five domains, a unique N-terminal domain L with no inhibitory effects and four homologous domains (CAST 1-4) that can inhibit heterodimeric calpain 1 and 2 (3).

Specifications