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Cell Signaling Sqle Antibody
List Price
$309.06
Your Price
$309.06
Cell Signaling Sqle Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-40659S
MFG.PART:
40659S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 60 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | SQLE Antibody recognizes endogenous levels of total SQLE protein. |
| Species Reactivity | Human |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro85 of human SQLE protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Squalene monooxygenase (SQLE), also known as squalene epoxidase, catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene (1,2) and is considered to be a critical rate-limiting enzyme in cholesterol biosynthesis downstream of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) (3). The N-terminal region of SQLE is anchored to the endoplasmic reticulum membrane via a re-entrant loop, and undergoes a conformational change in response to elevated cholesterol levels. This cholesterol-mediated conformational change promotes the interaction of SQLE with the E3 ligase MARCH6, leading to targeted ubiquitination and proteasomal degradation (4-6). Altered expression of SQLE is associated with perturbed cholesterol homeostasis and tumor progression, prompting investigation of the therapeutic potential of SQLE (7). Moreover, research studies have shown that cholesterol auxotrophy and subsequent squalene accumulation observed in certain cancers may prevent oxidative cell death and represent a targetable vulnerability to SQLE inhibitors (8,9). |
| SKU | CSIG-40659S |
|---|---|
| Featured | No |
| Supplier Part Number | 40659S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |