Cell Signaling Phospho-Dck (Ser74) Antibody

Name: Cell Signaling Phospho-Dck (Ser74) Antibody
SKU: CSIG-40371S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Dck (Ser74) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-40371S

MFG.PART: 40371S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	30
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-dCK (Ser74) Antibody recognizes endogenous levels of dCK protein only when phosphorylated at Ser74.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser74 of human dCK protein. Antibodies are purified by peptide affinity chromatography.
Background	Deoxycytidine kinase (dCK) catalyzes the rate-limiting step in the nucleoside salvage pathway. dCK phosphorylates salvaged deoxycytidine (dC) to generate deoxycytidine monophosphate (dCMP), a precursor for both dCTP and dTTP pools (1). dCK also phosphorylates deoxyadenosine (dA) and deoxyguanosine (dG) to generate deoxyadenosine monophosphate and deoxyguanosine monophosphate, respectively. Gemcitabine is an anticancer nucleoside that is a dC analog. It is a pro-drug and must be phosphorylated by dCK to an active form. dCK is phosphorylated on Ser74 by the DNA damage signaling kinases ATM and ATR, and this phosphorylation increases dCK activity (2,3).

Specifications