Cell Signaling Grb2 Antibody

Name: Cell Signaling Grb2 Antibody
SKU: CSIG-3972S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Grb2 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3972S

MFG.PART: 3972S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Hm Mk B
Sensitivity	Endogenous
Molecular Weight (kDa)	25
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GRB2 Antibody detects endogenous levels of total GRB2 protein.
Species Reactivity	Human, Mouse, Rat, Hamster, Monkey, Bovine
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Growth factor receptor-binding protein 2 (GRB2) is an adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1 (1). The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of Tyr209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling (2).

Specifications