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Cell Signaling Ilk1 (4g9) Rabbit mAb
List Price
$402.99
Your Price
$402.99
Cell Signaling Ilk1 (4g9) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-3856S
MFG.PART:
3856S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Hm Mk Z B Pg |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 51 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:100 - 1:400} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | ILK1 (4G9) Rabbit mAb detects endogenous levels of total ILK1 protein. The antibody may cross-react with ILK2. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, Pig |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ILK1. |
| Background | Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2). |
| SKU | CSIG-3856S |
|---|---|
| Featured | No |
| Supplier Part Number | 3856S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |