Cell Signaling Cox Iv (D6i4k) Rabbit mAb (Rodent Specific)

Name: Cell Signaling Cox Iv (D6i4k) Rabbit mAb (Rodent Specific)
SKU: CSIG-38563S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Cox Iv (D6i4k) Rabbit mAb (Rodent Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-38563S

MFG.PART: 38563S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	M R
Sensitivity	Endogenous
Molecular Weight (kDa)	17
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:800}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	COX IV (D6I4K) Rabbit mAb recognizes endogenous levels of total rodent COX IV protein. Weak staining was observed in some human tissues by immunohistochemstry.
Species Reactivity	Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp171 of mouse COX IV protein.
Background	Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

Specifications