Cell Signaling E2f-1 Antibody

Name: Cell Signaling E2f-1 Antibody
SKU: CSIG-3742S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling E2f-1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3742S

MFG.PART: 3742S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	70
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Chromatin IP: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	E2F1 Antibody detects endogenous levels of total E2F1 protein. The antibody does not cross-react with other proteins.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human E2F1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The E2F transcription factors are essential for regulation of the cell cycle (1,2). Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit (3, 4). Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control (5-8). E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases (9-11).

Specifications