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Cell Signaling Cleaved N4bp1 (Asp488) (E8x4w) Rabbit mAb
List Price
$435.31
Your Price
$435.31
Cell Signaling Cleaved N4bp1 (Asp488) (E8x4w) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-37378S
MFG.PART:
37378S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Cleaved N4BP1 (Asp488) (E8X4W) Rabbit mAb recognizes endogenous levels of N4BP1 protein only when cleaved at Asp488. A non-specific band has been observed at 62 kDa by Western Blot. |
| Species Reactivity | Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp488 of mouse N4BP1 protein. |
| Background | N4BP1 (NEDD4-binding protein 1) was identified as a binding partner and substrate for the HECT domain-containing ubiquitin ligase NEDD4 (1). Subsequently, it was also found to interact with Itch, a ubiquitin ligase structurally similar to NEDD4 (2). Interaction between N4BP1 interferes with the ability of Itch to bind its substrates, including c-Jun and p63 (2). N4BP1 expression has been associated with immune regulation as well as cancer. N4BP1 was identified as an inhibitory factor of NF-κB-mediated MHC-1 expression in neuroblastoma and high expression of N4BP1 was linked to poor overall survival (3). Studies have also found that N4BP1 is a suppressor of cytokine production that can be cleaved and inactivated by caspase-8 (4). N4BP1 can interact with the NF-κB modulator NEMO to suppress NF-κB transcriptional activity (5). Studies have also identified N4BP1 as a potent interferon-inducible HIV-1 restriction factor that targets the degradation of viral mRNA (6). |
| SKU | CSIG-37378S |
|---|---|
| Featured | No |
| Supplier Part Number | 37378S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |