Cell Signaling Eef2k Antibody

Name: Cell Signaling Eef2k Antibody
SKU: CSIG-3692S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Eef2k Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3692S

MFG.PART: 3692S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	105
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200, Immunofluorescence (Immunocytochemistry): 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	eEF2k Antibody detects endogenous levels of total eEF2k independent of phosphorylation.
Species Reactivity	Human, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the N-terminus of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.

Specifications