Cell Signaling Cre Recombinase (D7l7l) Xp® Rabbit mAb (Alexa Fluor® 488 Conjugate)

Name: Cell Signaling Cre Recombinase (D7l7l) Xp® Rabbit mAb (Alexa Fluor® 488 Conjugate)
SKU: CSIG-36176S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Cre Recombinase (D7l7l) Xp® Rabbit mAb (Alexa Fluor® 488 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-36176S

MFG.PART: 36176S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	All
Sensitivity	Transfected only
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunofluorescence (Frozen): 1:100, Immunofluorescence (Immunocytochemistry): 1:100, Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Cre Recombinase (D7L7L) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes transfected and transgenic levels of total Cre recombinase protein.
Species Reactivity	All Species Expected
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein.
Background	Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).

Specifications