Cell Signaling Pde5 (P434) Antibody

Name: Cell Signaling Pde5 (P434) Antibody
SKU: CSIG-3585S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Pde5 (P434) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3585S

MFG.PART: 3585S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	100
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	PDE5 (P434) Antibody detects endogenous levels of total PDE5 protein. The antibody cross-reacts with a 35-40 kDa protein of unknown origin.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human PDE5.
Background	The superfamily of phosphodiesterases (PDEs) catalyses the hydrolysis of 3,5-cyclic nucleotides into the corresponding nucleotide 5-monophosphates. PDE5 is a cGMP-specific enzyme that contains two cGMP binding sites in its amino-terminal regulatory domain (1). Elevation of cGMP levels causes activation of PKG, which phosphorylates PDE5 at Ser102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, leading to a decrease in intracellular cGMP levels (3,4).

Specifications