Cell Signaling Bit1 Antibody

Name: Cell Signaling Bit1 Antibody
SKU: CSIG-3543S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Bit1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3543S

MFG.PART: 3543S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	19, 21
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Bit1 Antibody detects endogenous levels of total human Bit1 protein. It also detects overexpressed bovine Bit1 and may detect overexpressed levels of mouse and rat Bit1.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within a central region of Bit1. Antibodies were purified by protein A and peptide affinity chromatography.
Background	Bit1 is a mitochondrial protein discovered as a regulator of anoikis, a process of apoptosis triggered by the loss of cell adhesion to the extracellular matrix (1,2). Like several other mitochondrial apoptotic regulators (Cytochrome c, Smac, HtrA2, AIF, EndoG), Bit1 is released into the cytoplasm during apoptosis (1,3). Cytoplasmic Bit1 associates with the Groucho family of transcriptional regulator AES (1,4). Interaction between Bit1 and AES promotes apoptosis, and this complex is suppressed by integrin mediated cell attachment.

Specifications