Cell Signaling Phospho-Npm (Thr95) Antibody

Name: Cell Signaling Phospho-Npm (Thr95) Antibody
SKU: CSIG-3517S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Npm (Thr95) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3517S

MFG.PART: 3517S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	38
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:50, Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-NPM (Thr95) Antibody detects endogenous levels of NPM only when phosphorylated at Thr95.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr95 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).

Specifications