Cell Signaling Cdk2 (E8j9t) Xp® Rabbit mAb (Bsa And Azide Free)

Name: Cell Signaling Cdk2 (E8j9t) Xp® Rabbit mAb (Bsa And Azide Free)
SKU: CSIG-34541SF
Price: 851.43 USD

List Price $851.43 Your Price $851.43

Cell Signaling Cdk2 (E8j9t) Xp® Rabbit mAb (Bsa And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-34541SF

MFG.PART: 34541SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µg
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	33
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #18048.
Specificity/Sensitivity	CDK2 (E8J9T) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CDK2 protein. In rodent cell lines, isoforms 1 and 2 of CDK2 run as two distinct bands by western blot.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro294 of human CDK2 protein.
Background	Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).

Specifications