Cell Signaling Gst (26h1) Mouse mAb (Alexa Fluor 647 Conjugate)

Name: Cell Signaling Gst (26h1) Mouse mAb (Alexa Fluor 647 Conjugate)
SKU: CSIG-3445S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling Gst (26h1) Mouse mAb (Alexa Fluor 647 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3445S

MFG.PART: 3445S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	All
Sensitivity	Transfected Only
Source/Isotype	Mouse IgG2a
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	GST-Tag (26H1) Mouse mAb (Alexa Fluor® 647 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.
Species Reactivity	All Species Expected
Source/Purification	Monoclonal antibody is produced by immunizing animals with a GST fusion protein. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

Specifications