Cell Signaling Camkii-Alpha Antibody

Name: Cell Signaling Camkii-Alpha Antibody
SKU: CSIG-3357S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Camkii-Alpha Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3357S

MFG.PART: 3357S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	50
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	CaMKII-α Antibody detects endogenous levels of total CaMKII-α. The peptide sequence used as the antigen is not conserved in CaMKII-β, -γ and -δ.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of CaMKII-α. Antibodies are purified by protein A and peptide affinity chromatography.
Background	CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

Specifications