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Cell Signaling Ascpf1/Cas12a (Strain Bv3l6 ) (E1u7c) Rabbit mAb (Bsa And Azide Free)
List Price
$851.43
Your Price
$851.43
Cell Signaling Ascpf1/Cas12a (Strain Bv3l6 ) (E1u7c) Rabbit mAb (Bsa And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-33209SF
MFG.PART:
33209SF
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µg |
| Reactivity | All |
| Sensitivity | Transfected Only |
| Molecular Weight (kDa) | 151 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {} |
| Storage | Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #19984. |
| Specificity/Sensitivity | AsCpf1/Cas12a (Strain BV3L6) (E1U7C) Rabbit mAb (BSA and Azide Free) recognizes transfected levels of total AsCpf1/Cas12a (Strain BV3L6) protein. This antibody does not cross-react with Cas9 (S. pyogenes), Cas9 (S. aureus), and FnCpf1/Cas12a (Strain U112) proteins. |
| Species Reactivity | All Species Expected |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu822 of Acidaminococcus sp. Cpf1/Cas12a (Strain BV3L6) protein. |
| Background | CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4). AsCpf1 (Strain BV3L6)/Cas12a is a Cpf1/Cas12a enzyme derived from Acidaminococcus sp. BV3L6 (5,6). |
| SKU | CSIG-33209SF |
|---|---|
| Featured | No |
| Supplier Part Number | 33209SF |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |