Cell Signaling K-Ras Antibody

Name: Cell Signaling K-Ras Antibody
SKU: CSIG-33197S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling K-Ras Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-33197S

MFG.PART: 33197S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	21
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	K-Ras Antibody recognizes endogenous levels of total K-Ras protein. This antibody cross-reacts with N-Ras in a K-Ras preferred manner, but does not cross-react with H-Ras or R-Ras protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val125 of human K-Ras protein. Antibodies are purified by peptide affinity chromatography.
Background	The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

Specifications