Cell Signaling Phospho-Pyk2 (Tyr402) Antibody

Name: Cell Signaling Phospho-Pyk2 (Tyr402) Antibody
SKU: CSIG-3291S
Price: 360.57 USD

List Price $360.57 Your Price $360.57

Cell Signaling Phospho-Pyk2 (Tyr402) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3291S

MFG.PART: 3291S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	116
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Pyk2 (Tyr402) Antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402. This antibody may cross-react with other phospho-tyrosine containing proteins.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr402 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).

Specifications