Cell Signaling Gbetal Antibody

Name: Cell Signaling Gbetal Antibody
SKU: CSIG-3227S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Gbetal Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3227S

MFG.PART: 3227S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	37
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GβL Antibody detects endogenous levels of total GβL protein.
Species Reactivity	Human, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln164 of human GβL. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).

Specifications