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Cell Signaling Ifn-Gamma (3f1e3) Mouse mAb
List Price
$339.36
Your Price
$339.36
Cell Signaling Ifn-Gamma (3f1e3) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-3159S
MFG.PART:
3159S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Recombinant protein |
| Molecular Weight (kDa) | 17 |
| Source/Isotype | Mouse IgG1 |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Peptide ELISA (DELFIA): 1:100} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | IFN-γ (3F1E3) Mouse mAb detects recombinant human IFN-γ. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with Ni-NTA purified recombinant human IFN-γ expressed in E. Coli. Antibodies were prepared from ascites. |
| Background | IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). Due to differences in the degree of glycosylation, there are 3 forms of IFN-γ, with approximate molecular weights of 25, 20, and 15.5 kDa by SDS-PAGE (5). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).IFN-γ, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system. Many signaling proteins including IL-2, FGF, and EGF induce the synthesis of IFN-γ. |
| SKU | CSIG-3159S |
|---|---|
| Featured | No |
| Supplier Part Number | 3159S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |