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Cell Signaling Phospho-C-Abl (Tyr412) (247c7) Rabbit mAb
List Price
$400.97
Your Price
$400.97
Cell Signaling Phospho-C-Abl (Tyr412) (247c7) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-2865S
MFG.PART:
2865S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 135 (c-Abl); 210 (Bcr-Abl) |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb detects endogenous Abl proteins only when phosphorylated at tyrosine 412. The antibody does not cross-react with other phosphorylated tyrosine kinases. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr412 of human c-Abl. |
| Background | The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). |
| SKU | CSIG-2865S |
|---|---|
| Featured | No |
| Supplier Part Number | 2865S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer Name | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |