Cell Signaling C/Ebpalpha (P42) Antibody

Name: Cell Signaling C/Ebpalpha (P42) Antibody
SKU: CSIG-2843S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling C/Ebpalpha (P42) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2843S

MFG.PART: 2843S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	45
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	C/EBPalpha (p42) Antibody detects endogenous levels of total p42 C/EBPalpha protein but not p30 C/EBPalpha.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human C/EBPalpha. Antibodies are purified by protein A and peptide affinity chromatography.
Background	CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).

Specifications