Cell Signaling Tyrosine Hydroxylase Antibody

Name: Cell Signaling Tyrosine Hydroxylase Antibody
SKU: CSIG-2792S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Tyrosine Hydroxylase Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2792S

MFG.PART: 2792S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	55-60
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Tyrosine Hydroxylase Antibody detects endogenous levels of total tyrosine hydroxylase.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9).

Specifications