Cell Signaling Bcl-Xl (54h6) Rabbit mAb

Name: Cell Signaling Bcl-Xl (54h6) Rabbit mAb
SKU: CSIG-2764T
Price: 146.45 USD

List Price $146.45 Your Price $146.45

Cell Signaling Bcl-Xl (54h6) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2764T

MFG.PART: 2764T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	30
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:1200, Immunofluorescence (Immunocytochemistry): 1:200, Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product #61060.
Specificity/Sensitivity	Bcl-xL (54H6) Rabbit mAb detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL.
Background	Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5).

Specifications