Cell Signaling Mgmt Antibody

Name: Cell Signaling Mgmt Antibody
SKU: CSIG-2739S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Mgmt Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2739S

MFG.PART: 2739S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	21
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MGMT Antibody detects endogenous levels of total MGMT protein.
Species Reactivity	Human, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human MGMT. Antibodies are purified by peptide affinity chromatography.
Background	MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O(6) position of guanine, restoring guanine to its normal form without causing DNA breaks (1). MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents (2,3). It is ubiquitously expressed in normal human tissues (4) and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy (5).

Specifications