Cell Signaling Beta-Catenin (L54e2) Mouse mAb (If Preferred)

Name: Cell Signaling Beta-Catenin (L54e2) Mouse mAb (If Preferred)
SKU: CSIG-2677S
Price: 339.36 USD

List Price $339.36 Your Price $339.36

Cell Signaling Beta-Catenin (L54e2) Mouse mAb (If Preferred) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2677S

MFG.PART: 2677S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	92
Source/Isotype	Mouse IgG1
Application/Dilution	{Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:200, Flow Cytometry (Fixed/Permeabilized): 1:200}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #34396.
Specificity/Sensitivity	β-Catenin (L54E2) Mouse mAb detects endogenous levels of total β-catenin protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β-catenin.
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Specifications